Biosynthesis of silver nanoparticles using water extracts of Nepeta cataria L. and Salvia sclarea L., characterization and determination of antibacterial potential

Abstract

Benefits of silver nanoparticles (AgNPs) against various pathogens and their other benefits are already well known in medicine. Meanwhile, applications involving nanotechnology have the potential to aid agricultural production. Bacteria, fungi, viruses that cause plant diseases can be controlled with AgNPs. Bacterial infections cause significant loss of yield around the world. AgNPs have shown activity against plant pathogenic bacteria. They have higher antibacterial activity against erwinia cartovora, E. than antibiotics. AgNPs also lave antifungal activity against pathogenic fungi, ie. Fusarium oxysporum, Alternaria Alternaria and Aspergillus flavus. They represent alternative to pesticides, are able to protect plant from pest and enrich soil with nutriants. Their use may reduce demand on chemical fertilizers. It is already used as foliar spray against moulds, rot, fungi and other microbial associated plant diseases . AgNPs can benefit preservation process of food. Their addition to food packaging can improve their barrier, mechanical, and antibacterial properties, and maintain the quality of foods. Biosynthesis is simple, effective, environmentally friendly and inexpensive way for production of silver nanoparticles. Process does not require high temperature and pressure. It is characterized by low energy costs. In given study silver nanoparticles were biosynthesized using water extracts of Nepeta Cataria L. (Catnip) and Salvia Sclarea L, (Clary Gramsage). Process of biosynthesis was observed and optimal conditions were determined. Morphology of obtained nanoparticles was investigated using TEM method and AgNPS were evaluated for antibacterial activity against ten stains of Gram-negative and Gram-positive bacteria.