Influence of Ca on the assimilation of Cr and Zn by the chromium resistant bacterium ARTHROBACTER GLOBIFORMIS 151B

Authors

  • A. Rcheulishvili Elefter Andronikasvili Institute of Physics, Ivane Javakhishvili Tbilisi State University, 6, Tamarashvili street, Tbilisi, 0172, Georgia
  • M. Gurielidze Sergi Durmishidze Institute of Biochemistry and Biotechnology, Agricultural University of Georgia, 240, David Agmashenebeli Alley, Tbilisi, 0159, Georgia
  • E. Ginturi Elefter Andronikasvili Institute of Physics, Ivane Javakhishvili Tbilisi State University, 6, Tamarashvili street, Tbilisi, 0172, Georgia
  • L. Tugushi Elefter Andronikasvili Institute of Physics, Ivane Javakhishvili Tbilisi State University, 6, Tamarashvili street, Tbilisi, 0172, Georgia
  • Hoi-Ying Holman Lawrence Berkeley National Laboratory (LBNL), University of California, USA

Keywords:

Bacteria (Arthrobacter globiformis 151B), Biomass, Metals, Concentration, Cultivation, Detoxification

Abstract

The process of assimilation of Cr(VI) and Zn by chromium-resistant bacteria (Arthrobacter globiformis 151B) and the influence of
high-concentration Ca ions on this process have been studied in the article. The bacteria are known for their property to assimilate intensively the hexavalent chromium [Cr(VI)] ions from the environment, to convert them into trivalent form [Cr(III)] and to accumulate it in cell. Thanks to these properties, it is possible to use them for detoxification of the environment polluted by highly toxic Cr(VI). The strain of bacteria under investigation was isolated from basalt samples, taken from the places highly contaminated by Cr(VI) in Kazreti. The solutions of the studied elements (Cr and Zn) and of Ca were introduced simultaneously into the nutrient medium. We studied the influence of different concentrations of Ca ions during different period of time of bacteria cultivation (17h, 24h, 48h, 96h, 144h) on the process of assimilation of Cr and Zn by bacteria. Ca concentration in nutrient medium made up 100 mcg/ml, 400 mcg/ml and 1600 mcg/ml.For determination of the content of Cr and Zn in the cell, after the cultivation of bacteria the precipitation of cells by centrifuge and the preparation of the obtained bacterial pellet for the analysis were carried out. The content of metals was measured by atom-absorption spectrometry.

Additional Files

Published

01-02-2021

How to Cite

Rcheulishvili, A., Gurielidze, M., Ginturi, E., Tugushi, L., & Holman, H.-Y. (2021). Influence of Ca on the assimilation of Cr and Zn by the chromium resistant bacterium ARTHROBACTER GLOBIFORMIS 151B. The Journal of Nature Studies - Annals of Agrarian Science, 18(3). Retrieved from https://journals.org.ge/index.php/aans/article/view/237

Issue

Vol. 18 No. 3 (2020): Annals of Agrarian Science

Section

Articles