Molecular characterization and phylogenetic relationships of Globodera rostochiensis isolates in two geographically distinct regions (Samckhet-Javakheti and Svaneti) of Georgia
Keywords:
Globodera rostochiensis, Molecular characterization, Phylogenetic tree, rDNA, D3 expansion region, Polymerase Chain reaction (PCR)Abstract
Molecular characterization of Globodera rostochiensi was performed by the amplification and sequencing D3 expansion region of the large subunit ribosomal gene (28S rDNA).Molecular analysis of G. rostochiensis had not been previously performed in Georgia. Sequence analyses of D3 expansion region of the rRNA using computer program BLASTN shown (94-99%) identity with D3 expansion region to all known isolates of G. rostochiensis. Based on analysis of G. rostochiensis isolates D3 expansion regions are grouped in three major clades ( A, B and C) on the phylogenetic tree.Clade A are divided in three subclades, clade C are divided in two subclades. Isolates from Samtckhet-javakheti population are in subclade 1 of clade A and in subclade 1 of clade C. Isolates from Svaneti populations are in subclade 2 of clade A and in clad B. In Clade C subclade 2 is presented by three isolates from Svaneti and by one isolates from Samckhet-Javakheti. Our investigation showed of high genetic variation of D3 region of rDNA of the isolates of G. rostochiensis in two geographically distinct origins (Samckhet-Javakheti and Svaneti) of Georgia.
